Monday, November 10, 2008

Gynogenesis

It is the process to produce individuals from maternal chromosomes only eventually to obtain homozygosity. Gynogenesis in fish farming is used to form inbred lines to achieve proper hybridization and selective breeding.

Methods:

Sperm nucleus is inactivated prior to fertilization by use of X-Rays, chemicals – dimethyl sulphate.
Upon fertilisation the resulting diploid individuals retain the second polar body nucleus (maternal) besides the egg nucleus, because the eggs are exposed to sub lethal temperature shocks before or after fertilization which suppress the meiotic divisions of eggs i.e. ensuring the non reduction of nuclear components.

In India Gynogenesis are tried on Indigenous as well as Exotic carps. Eggs of rohu are fertilised with irradiated sperms of Catla and then exposed to cold 12°C and heat 39°C shocks to obtain gynogenetic rohu.

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